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mcherry fkbp pten  (Addgene inc)


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    Structured Review

    Addgene inc mcherry fkbp pten
    Mcherry Fkbp Pten, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+fkbp+pten/mCherry-FKBP-PTEN+(Plasmid+%23116866)/pm41912541-495-26-36
    Average 92 stars, based on 4 article reviews
    mcherry fkbp pten - by Bioz Stars, 2026-10
    92/100 stars

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    Generated:

    Article Title: Lipid turnover and GEF recruitment collectively determine Rab5 recruitment and activation during the first step of early endosome formation.
    Article Snippet: .. The plasmids LYN11-FRB-ECFP (#38003), mCherry-FKBP-INPP4B (#116864), NES-EGFPcPHx3 (#116855), and mCherry-FKBP-PTEN (#116866) were obtained from Addgene. mCherryFKBP-ptaseOCRL, mCherry-FKBP-ptaseOCRL(D523G), mCherry-FKBP-Sac1, and mCherryFKBP-Sac1(C392S) were generated by replacing PTEN in mCherry-FKBP-PTEN (#116866) with the 5-phosphatase domain of OCRL from mCherry-CRY2-5ptaseOCRL (Addgene, #66836), the D523G mutant of the 5-phosphatase domain of OCRL from mCherry-CRY2-5ptaseOCRL(D523G) (Addgene, #66837), Sac1 from PJ-Sac (Addgene, #38000), or Sac1(C392S) from PJ-DEAD (Addgene, #38002). .. The plasmids LYN11-FRB-ECFP (#38003), mCherry-FKBP-INPP4B (#116864), NES-EGFPcPHx3 (#116855), and mCherry-FKBP-PTEN (#116866) were obtained from Addgene.

    Mutagenesis:

    Article Title: Lipid turnover and GEF recruitment collectively determine Rab5 recruitment and activation during the first step of early endosome formation.
    Article Snippet: .. The plasmids LYN11-FRB-ECFP (#38003), mCherry-FKBP-INPP4B (#116864), NES-EGFPcPHx3 (#116855), and mCherry-FKBP-PTEN (#116866) were obtained from Addgene. mCherryFKBP-ptaseOCRL, mCherry-FKBP-ptaseOCRL(D523G), mCherry-FKBP-Sac1, and mCherryFKBP-Sac1(C392S) were generated by replacing PTEN in mCherry-FKBP-PTEN (#116866) with the 5-phosphatase domain of OCRL from mCherry-CRY2-5ptaseOCRL (Addgene, #66836), the D523G mutant of the 5-phosphatase domain of OCRL from mCherry-CRY2-5ptaseOCRL(D523G) (Addgene, #66837), Sac1 from PJ-Sac (Addgene, #38000), or Sac1(C392S) from PJ-DEAD (Addgene, #38002). .. The plasmids LYN11-FRB-ECFP (#38003), mCherry-FKBP-INPP4B (#116864), NES-EGFPcPHx3 (#116855), and mCherry-FKBP-PTEN (#116866) were obtained from Addgene.

    other:

    Article Title: New evidence for the presence and function of phosphoinositides (PPIs) in the chloroplast
    Article Snippet: In brief, the coding sequence of the lipid binding domains of the pleckstrin homology (PH) domain of human ARHGEF9 for PI5P binding, switch-associated protein 70 PH domain of human differentially expressed in FDCP6 homolog guanidine exchange factor DEF6 for PI3,4P detection, the PH domain of human rho guanidine nucleotide exchange factor 7 (ARHGEF7) for PI3,5P detection , the four phosphate adaptor protein 1 and 2 (PH) domain of rat FAPP1 for PI4P detection, the PH domain of mouse 1-phosphatidylinositol-4,5-bisphosphate diesterase delta (Plcd1) for PI4,5P detection and putative phosphatidylinositol 3-phosphate binding site encoded by the FYVE domain from human hepatocyte growth factor related tyrosine kinase substrate (HGS11) ( ) was codon optimized for expression in Arabidopsis were codon optimized and synthesized with recombination sites (sequences in supplement) to be integrated into pDK366 containing cTP_YFP, cTP_mCherry or cTP_mEOS4b fusion proteins under the control of the 35S promotor by InFusion clonng., mCherry-FKBP-PTEN was a gift from Gerry Hammond (Addgene plasmid # 116866; http://n2t.net/addgene:116866 ; RRID:Addgene_116866), SAC7/RHD4 (At3g51460) was amplified from Arabidopsis thaliana cDNA. dOCRL was used as codon optimized synthetic DNA of the sequence publishes in .



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